3D calibration standards
3D calibration for SEM, AFM and CLSM
The 3D calibration structures consist of an array of multi-level individual pyramids. There are ring-shaped reference marks (nanomarkers) on the calibration structure, the centers of which are measured with high precision. These enable the determination of the scales and couplings (shears) of all three coordinate axes of the measuring device used.
For each calibration structure you will receive a file with the associated reference information. By using coded reference marks, the calibration structures are suitable for automated calibration processes. To do this, use our 3D calibration kit with the calibration software microCal.
The 3D calibration structures can be used universally (3D SEM, AFM, CLSM). Other variants of these calibration structures as well as customer-specific adjustments are possible on request.
3D calibration structure - design
The 3D calibration structure consists of an array of:
3 multi level single pyramids
1 spherical element for the calibration of the four backscatter detectors (diameter: 10 µm, height: ca. 1µm)
reference marks (nanomarker)
The calibration structure includes circular nanomarker with exactly measured centre coordinates (reference information).
In combination with our 3D calibration software microCal, the calculation of all three scales of your 4Q-BSE-detector system can easily be achieved.
We provide the reference information as a data file together with the 3D calibration structure.
Advantages
One Step
Calibrate lateral and vertical calibration simultaneously in one measurement step.
One Model
Enable determination of coupling between vertical and lateral axes.
One Click
Benefit from extremely efficient and accurate calibration due to advanced image processing methods.
One Reference
Process universal applications with one reference structure for SPM, CLSM, SEM or other.
Calibration samples
- FIB-SEM deposited 3D structures with reference marks for automated calibration
- For SEM, AFM and optical 3D systems
- 3x pyramids for calibration in X, Y and Z directions
- 1x spherical element for adjustment of 4Q BSE detectors
Calibration data
- Calibrated 3D reference data acquired with a metrological SPM system
- Used for comparison of recorded and reference 3D data
- Prepared for automated calibration with calibration software
- Supplied on a USB drive
Calibration software
- Automated, statistical 3D calibration algorithm
- Determines scales in X, Y, Z and all shear factors
- Gives corrections parameters for acquisition system or pre-recorded data
- Single user license for Windows 11 ... 7
3D calibration standard - further details
Calibration structures
- 3 multi-level pyramidal elements
- 1 spherical element
- Reference marks
Pyramidal elements
- Produced by FIB deposition
- 3 with nominal size of 20 × 20 × 3 µm
Spherical element
- Produced by FIB deposition
- 10 × 10 × 1 μm (nominal)
Auto-recognition elements
- Produced by FIB milling
- 800 nm diameter (nominal)
Calibration area
- 80 × 80 µm
- 40 × 40 µm (single pyramid)